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qiaamp circulating nucleic acid kit  (Qiagen)


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    Structured Review

    Qiagen qiaamp circulating nucleic acid kit
    Qiaamp Circulating Nucleic Acid Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleic+acid+kit/QIAamp+Circulating+Nucleic+Acid+Kit/pm42882841-90-10-15
    Average 99 stars, based on 4575 article reviews
    qiaamp circulating nucleic acid kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Evaluation of ct-DNA and mutational status in liquid biopsy of patients with metastatic colorectal cancer
    Article Snippet: Peripheral blood samples were collected into Cell-Free DNA BCT tubes (Streck LLC.), particularly designed to preserve cfTNA. .. Plasma was obtained through two sequential centrifugations at 447 × g for 10 min at 4°C to ensure complete removal of cellular components. cfTNA was then extracted from 2 ml plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Inc.), according to the manufacturer's instructions. ..

    Article Title: Integrated targeted sequencing reveals unique tissue-of-origin and donor-derived cell-free DNA signatures in stable organ transplant recipients
    Article Snippet: .. For cfDNA isolation, the plasma was thawed at RT and isolated from 5 mL using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany). .. DNA concentration was measured using the Qubit dsDNA 1X HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Combined ctDNA and serum PSA for dynamic monitoring of metastatic prostate cancer starting first-line treatment: a prospective national cohort study.
    Article Snippet: .. For all samples, ccfDNA was extracted from 4–10 ml of plasma using the QIAamp Circulating Nucleic Acid kit (Qiagen) as per the manufacturer’s instructions, with the exception of the incubation period at 60 °C, which was extended to 1 h instead of 30 min. Germline DNA was extracted from 0.2 ml of buffy coat using QIAamp DNA Blood Mini Kit (Qiagen) as per the manufacturer’s instructions. ..

    Article Title: A non-invasive circulating tumor DNA methylation classifier to identify benign pulmonary nodules
    Article Snippet: .. C C R - 2 5 - 4 5 2 0 / 3 7 9 3 5 3 4 / c c r - 2 5 - 4 5 2 0 . p d f b y g u e s t o n 1 5 M a y 2 0 2 6 For tissue samples, fresh tumor and adjacent normal tissues were collected during surgery and stored at –80°C prior to processing. cfDNA was extracted from 2-4 mL of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Germany) according to the manufacturer’s instructions. .. Genomic DNA (gDNA) was extracted from tissue and blood cell samples using the DNeasy Blood & Tissue Kit (Qiagen, Germany).

    Article Title: Quantitative BRAF p.V600E mutation monitoring in cerebrospinal fluid cell-free DNA reflects therapeutic response to BRAF/MEK inhibitors in papillary craniopharyngioma: a report of two cases.
    Article Snippet: .. At the time of analysis, cfDNA was extracted from 1.0 mL of CSF samples using the QIAamp Circulating Nucleic Acid kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions. ..

    Isolation:

    Article Title: Integrated targeted sequencing reveals unique tissue-of-origin and donor-derived cell-free DNA signatures in stable organ transplant recipients
    Article Snippet: .. For cfDNA isolation, the plasma was thawed at RT and isolated from 5 mL using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany). .. DNA concentration was measured using the Qubit dsDNA 1X HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Disrupted neuronal expression of autism risk genes and white matter micro-organization after infant Zika infection.
    Article Snippet: 450 ZIKV-RNA detection by qRT-PCR: Total RNA was extracted from 140 μl of plasma using the 451 QIAamp Viral RNA Mini Kit (Qiagen). .. RNA from urine was isolated using the QIAamp 452 Circulating Nucleic Acid Kit (Qiagen). .. ZIKV-RNA standard was generated by annealing two 453 oligonucleotides spanning target ZIKV prM-E gene region and performing in vitro transcription 454 using the MEGAscript T3 Transcription Kit (Ambion).

    Article Title: Sensitivity and prognostic significance of circulating tumor DNA (ctDNA) in stage I to III malignant melanoma.
    Article Snippet: .. Isolation and quantitation of ctDNA from serum CtDNA was purified from 2 ml of serum using the QIAamp Circulating Nucleic Acid kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. DNA quantity was measured using the Bioanalyzer High Sensitivity DNA Reagents kit (Agilent Technologies Inc., Santa Clara, United States) following the manufacturer ́s instructions.

    Incubation:

    Article Title: Combined ctDNA and serum PSA for dynamic monitoring of metastatic prostate cancer starting first-line treatment: a prospective national cohort study.
    Article Snippet: .. For all samples, ccfDNA was extracted from 4–10 ml of plasma using the QIAamp Circulating Nucleic Acid kit (Qiagen) as per the manufacturer’s instructions, with the exception of the incubation period at 60 °C, which was extended to 1 h instead of 30 min. Germline DNA was extracted from 0.2 ml of buffy coat using QIAamp DNA Blood Mini Kit (Qiagen) as per the manufacturer’s instructions. ..

    Quantitation Assay:

    Article Title: Sensitivity and prognostic significance of circulating tumor DNA (ctDNA) in stage I to III malignant melanoma.
    Article Snippet: .. Isolation and quantitation of ctDNA from serum CtDNA was purified from 2 ml of serum using the QIAamp Circulating Nucleic Acid kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. DNA quantity was measured using the Bioanalyzer High Sensitivity DNA Reagents kit (Agilent Technologies Inc., Santa Clara, United States) following the manufacturer ́s instructions.

    Purification:

    Article Title: Sensitivity and prognostic significance of circulating tumor DNA (ctDNA) in stage I to III malignant melanoma.
    Article Snippet: .. Isolation and quantitation of ctDNA from serum CtDNA was purified from 2 ml of serum using the QIAamp Circulating Nucleic Acid kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. DNA quantity was measured using the Bioanalyzer High Sensitivity DNA Reagents kit (Agilent Technologies Inc., Santa Clara, United States) following the manufacturer ́s instructions.



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